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Objective:To analyze the CT manifestations of the 2019 novel coronavirus pneumonia (NCP) combined with severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) literature review, and to summarize the characteristics of CT imaging, so as to improve the ability of rapid and accurate diagnosis.Methods:CT manifestations of two confirmed cases of NCP were reported, meanwhile the literatures on SARS and MERS imaging performance were reviewed and summarized.Results:The two cases of NCP were both in acute stage, the CT imaging showed multiple and scattered ground-glass opacity (GGO) in both lungs, which is similar to the CT performance of SARS and MERS in acute stage.Conclusions:The CT features of 2019 novel coronavirus pneumonia are similar to SARS and MERS. It has certain characteristics and changes rapidly with the course of the disease. In the acute stage, GGO and paving stone sign were the main manifestations. In the acute phase, GGO and crazy paving are the main manifestations. In the progress stage, the interlobular septal thickening and consolidation appeared. During the absorption period, the lesions disappeared or fibrosis was left behind, with lung structure distortion and bronchiectasis. Lymphadenopathy and hydrothorax were rare.
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Objective@#To analyze the CT manifestations of the 2019 novel coronavirus pneumonia (NCP) combined with severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) literature review, and to summarize the characteristics of CT imaging, so as to improve the ability of rapid and accurate diagnosis.@*Methods@#CT manifestations of two confirmed cases of NCP were reported, meanwhile the literatures on SARS and MERS imaging performance were reviewed and summarized.@*Results@#The two cases of NCP were both in acute stage, the CT imaging showed multiple and scattered ground-glass opacity (GGO) in both lungs, which is similar to the CT performance of SARS and MERS in acute stage.@*Conclusions@#The CT features of 2019 novel coronavirus pneumonia are similar to SARS and MERS. It has certain characteristics and changes rapidly with the course of the disease. In the acute stage, GGO and paving stone sign were the main manifestations. In the acute phase, GGO and crazy paving are the main manifestations. In the progress stage, the interlobular septal thickening and consolidation appeared. During the absorption period, the lesions disappeared or fibrosis was left behind, with lung structure distortion and bronchiectasis. Lymphadenopathy and hydrothorax were rare.
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Objective To investigate two enteral nutrition injection methods on prognosis of patients with acute cerebral infarction (ACI) induced pseudobulbar palsy patients. Methods A total of 86 patients of ACI accompanied with dysphagia were randomly divided into the feeding pump group (n=43) and the traditional nasal feeding group (n=43). Nutritional support for both groups lasted at least more than 14 d through the feeding tube. In the first and fourteenth day data of both groups concerned with body mass index (BMI), triceps skin fold (TSF), upper arm muscle circumference of triceps (MAMC), heamoglobin (Hb), serum prealbumin (Alb), alanine transaminase(ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine (Cr), incidence of complications, neuro-function score NIHSS and the swallowing function evaluation were collected and analyzed. Results Nutritional index Hb and Alb was (132.6±10.1) g/L and (38.2±1.4) g/L in the group of the feeding pump group, and significantly higher than (122.2±13.2) g/L and (36.3±1.5) g/L in the traditional nasal feeding group in the fourteenth day, the difference was significant (t=12.86, 12.37, P<0.05). The NHISS score was 5.2±1.0 in the feeding pump group, significantly lower than 8.9±1.1 of the traditional nasal feeding group, the difference was significant (t=-26.37, P < 0.05). The improved rate of swallowing function was 81.4% (35/43) in the feeding pump group, significantly higher than 60.5%(26/43) of the traditional nasal feeding group, the difference was significant (χ2=4.568, P < 0.05). Conclusions The use of enteral feeding pump at early stage for ACI patients with pseudobulbar palsy can improve the nutritional status, acute neurological function, swallowing function and lower the incidence of complications which is beneficial for early recovery and short-term prognosis.
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BACKGROUND:Previous studies have confirmed that as an exogenous origin of endometrial epithelial cel s, bone marrow stem cel s are involved in the functional reconstruction of the injured endometrium. OBJECTIVE:To discuss whether bone marrow mesenchymal stem cel s can differentiate into endometrial epithelial cel s in vitro. METHODS:Passage 2 bone marrow mesenchymal stem cel s and endometrial stromal cel s were col ected and subjected to different treatments:bone marrow mesenchymal stem cel s were cultured alone in the DMEM/F12 medium containing 2%fetal bovine serum or in the DMEM/F12 medium containing 2%fetal bovine serum, 10-7 mol/Lβ-estradiol and 10μg/L epidermal growth factor as groups 1 and 2, respectively;bone marrow mesenchymal stem cel s co-cultured with endometrial stromal cel s by Transwel chamber were cultured in the DMEM/F12 medium containing 2%fetal bovine serum or in the DMEM/F12 medium containing 2%fetal bovine serum, 10-7 mol/Lβ-estradiol and 10μg/L epidermal growth factor as groups 3 and 4, respectively. After 5 days culture, bone marrow mesenchymal stem cel s at the bottom of the culture plate were col ected to detect expressions of epithelial cel markers, such as CK7, CK18, CK1 and EMA by RT-PCR technology, and to determine keratin expression using immunofluorescence assay. Besides, expressions of these epithelial cel markers in the group 3 were detected using RT-PCR at 1, 3, 5 and 7 days of culture, respectively. RESULTS AND CONCLUSION:mRNA expression of CK7 showed a significant successive rise in the groups 1, 2, 3, 4 and it was highest in the group 4. mRNA expressions of CK18 and CKl9 in the later three groups had no significant differences, but al significantly higher than those in the group 1. Compared with the groups 1 and 3, mRNA expression of EMA in the groups 2, 4 were significantly higher, but no significant differences existed between groups. Expression of keratin was strongly positive in the group 4, weakly positive in the group 3 and negative in the groups 1, 2, respectively. Furthermore, with the increase of culture time, mRNA expression of CK7 exhibited a constant increase, which was significantly higher at 5 and 7 days than at 1 and 3 days;but there were no significant differences in expressions of CKl9, CKl8 and EMA at different time points. These results show that bone marrow mesenchymal stem cel s can differentiate into endometrial epithelial cel s in vitro under certain conditions. Moreover, it can remarkably promote the endometrial differentiation under the combined effects of some exogenous and endogenous factors.
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Objective To study the therapeutic effects of human umbilical cord mesenchymal stem cells (hUCMSCs) on acute liver failure ( ALF) induced by D-galactosamine (D-gal) and lipopolysaccharide (LPS) in rats. Methods The ALF model was obtained through intraperitoneal injection of D-gal(300 mg/kg)and LPS (20μg/kg)in Wister rats. The hUCMSCs were transplanted after intoxication. All rats were divided into four groups, and each group received either hUCMSCs or 0.9% NaCl solution through intraperitoneal or tail-intravenous injection. To evaluate the liver function of each group, the levels of alanine aminotransferase (ALT), total bilirubin (TBil) and serum albumin (Alb) were measured on the day of hUCMSCs transplantation and the following 1, 2, 3, 5 and 7 days. All rats were then sacrificed to examine the liver histology at day 7. Analyses were done by using Fisher's exact test, unpaired t test and Mann-Whitney U test. Results There were no significant differences of survival rates among four groups (Fisher's exact test, both P = 1. 00). The levels of ALT, TBil and Alb in group receiving hUCMSCs intraperitoneally were (804. 9 ± 88. 0) U/L,(17. 4±2. 7) μmol/L and (20. 9±0. 8) g/L, respectively after 2 days of injection, whereas in the corresponding control group, those were (1294. 3± 171. 4) U/L, (32. 3±5. 5) μmol/L and (16. 1±0. 9) g/L, respectively, which indicated that hUCMSCs transplantation significantly improved the liver function (t = 2. 640, P =0.020;t=2.529, P = 0. 025;t= - 3. 833, P = 0. 002). Both of hUCMSCs-transplanted groups showed no significant differences. Liver histological data showed that transplantation of hUSMSCs through either intraperitoneal or tail-intravenous injection alleviated liver damage (U=4. 500, P = 0. 005;U=4. 500, P = 0. 008) and the mitotic index also increased in hUCMSCs-transplanted groups (U=4. 000, P = 0. 005; U=5. 500, P = 0. 013). Conclusions The levels of ALT, TBil and Alb can rapidly normalize in ALF rats after injected with hUCMSCs either intraperitoneally or tail-intravenously. hUCMSCs application raises the mitotic index, enhances hepatocellular regeneration and improves histological status.